演講與論壇

前瞻醫療器材科技專題演講 

 

演講者:日本北海道大學Toshiro Ohashi教授
演講時間:2012年11月22日(四)下午1:30至3:00
演講地點:社科院大樓心理系2樓階梯教室 
演講題目:Mechanical Characterization of Vascular Endothelial Cells Exposed to Fluid Shear Stress
主辦單位:成大前瞻醫療器材科技中心

Abstract
Vascular endothelial cells in vivo are exposed to complex mechanical forces including fluid shear stress, cyclic stretch and hydrostatic pressure. These mechanical forces are important factors in endothelial cell remodeling, possibly altering endothelial cell physiological functions. After applying fluid shear stress, cultured endothelial cells show marked elongation and orientation in the direction of flow. In addition, thick stress fibers of actin filaments develop and align along the long axis of cells. It is believed that cell deformations and associated mechanotransduction responses to mechanical forces are critical for cell-involved tissue homeostasis in health and disease. So far, a lot of efforts have been done to study the effects of mechanical stimuli on endothelial cell remodeling focusing on morphological and cytoskeletal changes or intracellular signaling events. However, little is still known of how mechanical forces are transmitted through cells to activate intracellular signaling cascades leading to alterations in cell functions. To further address this issue, it would be required to know intracellular mechanical environment such as mechanical properties of subcellular structural components including actin filaments, nucleus, etc. Cellular traction forces would also be interesting to know force transmission at focal adhesions. The objective of this talk is to present recent findings regarding mechanical characteristics of sheared endothelial cells by performing several mechanical tests including atomic force microscopy (AFM), micropipette aspiration tests, and traction force microscopy.